Table 1.
Real-Time PCR Quantification of mRNA Levels in HSCs Isolated from Control and CCl4-Treated DDR2−/− and DDR2+/+ Livers
| mRNA | Oil |
CCl4 |
||
|---|---|---|---|---|
| +/+ | −/− | +/+ | −/− | |
| Osteopontin | 1.0 ± 0.025 | 5.7 ± 0.003⁎ | 8.1 ± 0.001⁎ | 15.4 ± 0.012⁎† |
| TGF-β | 1.0 ± 0.003 | 4.8 ± 0.030⁎ | 2.7 ± 0.025⁎ | 12.5 ± 0.020⁎† |
| IL-10 | 1.0 ± 0.050 | 1.9 ± 0.014⁎ | 0.8 ± 0.004 | 0.4 ± 0.003⁎† |
| Collagen I α1 | 1.0 ± 0.040 | 0.5 ± 0.125 | 3.5 ± 0.213⁎ | 2.1 ± 0.198⁎† |
| MMP-2 | 1.0 ± 0.210 | 0.7 ± 0.050⁎ | 10.6 ± 2.050⁎ | 3.2 ± 0.512⁎† |
| MMP-13 | 1.0 ± 0.060 | 0.8 ± 0.090 | 3.1 ± 0.048 | 1.5 ± 0.290⁎† |
N = 16 mice from 2 independent treatments, 2 mice per group. Relative mRNA expression by HSCs from control (oil-treated) DDR2+/+ livers compared to RPL13 mRNA expression: osteopontin: 1.29 ± 0.01; TGF-β: 0.18 ± 0.01; IL-10: 0.71 ± 0.004; collagen I α1: 1.0 ± 0.024; MMP-2: 0.04 ± 0.008; MMP-13: 0.01 ± 0.000.
⁎
P < 0.05 compared to HSCs from control DDR2+/+ livers.
†
P < 0.05 compared to HSCs from DDR2+/+ livers after CCl4 administration.