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. 2012 Jan 16;209(1):11–17. doi: 10.1084/jem.20112078

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© 2012 Bednarski et al.

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Figure 1. — Akt and Pim1 promote IL-7-dependent survival. (A) Cell death assessed by 7AAD uptake in wild-type pre–B cells cultured in IL-7 (+IL-7) and 48 h after IL-7 withdrawal (−IL-7). (B) Western blot analysis of Pim1, phosphorylated Foxo1 (p-Foxo1), phosphorylated Akt (p-Akt), and total Akt in whole cell lysates from wild-type pre–B cells in IL-7 (+) and 48 h after IL-7 withdrawal (−). Gapdh is shown as a protein loading control. Numbers represent band intensity relative to +IL-7 and standardized to loading controls. (C) Cell death assessed by 7AAD uptake in wild-type (Pim1^+/+) and Pim1^−/− pre–B cells cultured for 48 h in media containing IL-7 and 5 µM Akt inhibitor VIII (+) or DMSO (−) for 48 h. Shown is the mean and standard deviation from three independent experiments. Data in A and B are representative of at least three independent experiments.