Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan 16;209(1):11–17. doi: 10.1084/jem.20112078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Bednarski et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — RAG DSBs support pre–B cell survival. (A) A schematic of the germline (GL) IgLκ locus and unrepaired Jκ1 and Jκ2 coding ends (CEs) are shown. Vκ and Jκ gene segments (rectangles) and their associated recombination sequences (triangles) are shown as are the relative positions of the EcoRI and SacI sites and the JκIII probe used for Southern blot analyses. Southern blot analysis of genomic DNA from RAG1^−/−:IgH:Bcl2, and Artemis^−/−:IgH:Bcl2 pre–B cells cultured in IL-7 (0 h) and after IL-7 withdrawal (wd, 48 h). Hybridizing bands generated by the GL IgLκ locus and Jκ1 and Jκ2 un-repaired CEs are indicated. (B) Western blot analysis of phosphorylated p53 (p-p53) after withdrawal of IL-7 for the indicated times. Gapdh is shown as a protein loading control. Numbers represent band intensity relative to Artemis^−/−:IgH:Bcl2 at time 0 h and standardized to loading controls. No quantitation is presented for RAG1^−/−:IgH:Bcl2 as no bands are present. (C) Western blot analysis of p-p53 after IL-7 withdrawal in Artemis^−/−:IgH:Bcl2 treated with vehicle (DMSO) or the ATM inhibitor KU55933. Gapdh is shown as protein loading control. Numbers represent band intensity relative to time 0 h and standardized to loading controls. (D) Viability assessed by 7AAD uptake after withdrawal of IL-7 for the indicated times. Shown is the mean and standard deviation for three replicates. **, P < 0.0001; *, P = 0.001. Data in A, B, and D are representative examples of at least three independent experiments. Data in C are representative of two independent experiments.