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. 2012 Jan 16;209(1):139–155. doi: 10.1084/jem.20101387

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Figure 6. — MOs generate an inflammatory CD103⁻ DC subset 3 after adoptive transfer into RAG^−/− colitic mice. (A) Strategy used for the induction of colitis in Rag^−/− mice, the adoptive transfer of purified MOs, and the analysis of MO progeny in the colon. (B) Quantification of the total number of colonic CD45⁺ and MHC-II^hi cells expressed as fold change in CD45RB^hi (colitic) versus CD45RB^hi+lo (control) animals. Results are mean ± SD of three independent experiments with at least five mice per group. (C and D) Representative FACS plots (C) and quantification (D) of subsets 1, 2, 3 CD103⁻, 3 CD103⁺, and 4 in the colon of colitic versus control mice 7 wk after transfer of CD45RB^hi or CD45RB^hi+lo CD4⁺ T cells, respectively. Results are mean ± SD of three independent experiments with at least five mice per group. *, P < 0.05; ***, P < 0.001. (E) CD11b/CD103 expression on subset 3 in colitic versus control mice. (F and G) Analysis of the colon of colitic mice 3 d after engraftment or not with 2–3 × 10⁶ purified bone marrow MOs. The data presented are representative of at least two separate experiments with three MO-transferred mice each. (H) Real-time RT-PCR analysis of cytokine mRNA expression in CD45.1⁺ (MO derived) colonic cells FACS sorted from recipient colitic mice 3 d after adoptive transfer of 3 × 10⁶ bone marrow MOs (closed bars). The mRNA profile of MO-derived cells sorted from noncolitic CD11cDTR → C57BL/6 chimeric mice (data duplicated from Fig. 5 G) has been plotted here for comparison purposes (open bars). Results are mean ± SD of two experiments with five to seven pooled colons/group.