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. 2012 Jan 16;209(1):139–155. doi: 10.1084/jem.20101387

Figure 7.

Figure 7.

CD103− subset 3 exhibits potent T cell priming capacities and induces the differentiation of IFN-γ–producing T cells in vitro. (A) Proliferation of OT-II cells after co-culture with graded doses of OVA protein–pulsed cLP subsets 1, 2, 3 CD103−, 3 CD103+, and 4 and control splenic (SPL) DCs and MPs. Proliferation was determined by thymidine incorporation assay after 4 d of culture. Data are mean ± SD and are representative of two experiments performed in triplicates. Subset 3 CD103− was compared with subset 1 or 2 and splenic MPs using an unpaired Student’s t test, and the less significant p-values were plotted (black statistics). Subset 3 CD103− was also compared with subset 3 CD103+ (green statistics), subset 4 (violet statistics), and splenic DCs (orange statistics). foxp3, IFN-γ, and IL-17A intracellular FACS staining in OT-II cells co-cultured for 5 d with FACS-sorted OVA-loaded colonic subsets 1, 2, and 3 (CD103− or CD103+) from WT mice. (B) Representative plots. (C) Quantification of the absolute numbers of live foxp3+, IFN-γ+, and IL-17A+ OT-II cells/well in the co-cultures. (D) Quantification of IFN-γ and IL-17A production by OT-II cells after 4.5 d of culture. (C and D) Data are mean ± SD of two separate experiments performed in triplicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001.