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. 2012 Jan 16;209(1):157–171. doi: 10.1084/jem.20101354

Figure 2.

Figure 2.

HMGN1 induction of leukocyte recruitment. (A) 8-wk-old female C57BL/6 mice (five mice/group) were injected i.p. with PBS alone or PBS containing the indicated amount of HMGN1. After 4 h, leukocytes in the peritoneal cavity were enumerated. Shown is the mean (±SD) number of leukocytes. *, P < 0.05 by ANOVA when compared with mice injected with PBS alone. Shown are the results of one experiment representative of three. (B) Two groups of female C57BL/6 mice (10 wk old; n = 5) were injected i.p. with PBS alone or PBS containing 1 µg HMGN1. After 4 h, leukocytes were harvested from the peritoneum and immunostained with either isotype-matched control antibodies or a combination of PE-conjugated anti–mouse CD11c, FITC-conjugated anti–mouse CD11b, and APC-conjugated anti–mouse B220. The indicated subpopulations of DCs were determined by flow cytometry and calculated as fold increase induced by HMGN1 over the PBS group. Shown are the results of one experiment representative of two.