Figure 3.

Histopathology of hNF-E2 tg mice. (A–D) Prussian blue staining of spleens of hNF-E2 tg mice (right) and control mice (left). Iron deposits stain blue. (E–H) Gomori’s silver staining of spleens of hNF-E2 tg mice (right) and control mice (left). Reticulin fibers stain black. (I–L) Elastica van Gieson stain of spleens of hNF-E2 tg mice (right) and WT mice (left). Elastic fibers and nuclei stain black, collagen stains red, and smooth muscle stains yellow. The increase in iron deposits, fibrosis, and neovascularization was seen in mice of both tg strains, 9 and 39 (n > 50 each), to the same extent. Bars: (A–D) 100 µm; (E–H) 25 µm; (I–L) 50 µm.