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. 2012 Jan 16;209(1):35–50. doi: 10.1084/jem.20110540

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© 2012 Kaufmann et al.

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Figure 9. — Chromatin modifications in hNF-E2 tg and WT mice. (A–D) Western blot analysis and densitometric quantification of histone H3 acetylation (A), histone H4 acetylation (B), H3K27me3 (C), and histone H3K4me3 (D) in BMs of WT and hNF-E2 tg mice (n = 3 and 6, respectively). 10 µg of protein was interrogated for the indicated modification of histone H3 or H4. The blot was stripped and reprobed with an antibody against total histone H3 or histone H4 to ensure equal loading. The ratio of modified histone H3 or H4 to total histone H3 or H4, respectively, was calculated for each animal. One untreated WT littermate was arbitrarily set at 100%, and all other animals were quantitated relative to this calibrator. All data include mice of both strains, 9 and 39; no differences were observed between the two tg strains. Horizontal lines indicate the mean. *, P < 0.05; **, P < 0.01.