Skip to main content
. 2012 Jan 16;209(1):123–137. doi: 10.1084/jem.20111009

Figure 3.

Figure 3.

Mo clearance. Cell exit and local death were studied as major contributors to cell clearance from the site of inflammation. (A) Bar graph shows number of exited CD45.2+ Mo in organs based on FCM; pie chart illustrates percentage of exited myeloid cells normalized to the number in the infarct (n = 5). (B) Left bar graph shows excited cell number in organs calculated using alternative scintillation data; pie chart illustrates the exit rate. The right bar graph reflects decay-corrected photon counts of the graft before and 24 h after heart transplantation. Experiment was done in duplicate, data are mean ± SEM. (C) Immunofluorescence of infarct tissue (DAPI, blue; TUNEL, green; CD11b, red). Pie charts indicate frequency of TUNEL signal in CD11b+ cells (arrows; n = 5 per group). (D) Analysis of cell death by DAPI FCM staining in the heart (n = 4 per group). (E) In vitro time lapse microscopy of representative splenic Mo to determine timing of cell death signals. Experiment was done in duplicates.