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. 2012 Jan 16;209(1):187–199. doi: 10.1084/jem.20110645

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© 2012 Schulz et al.

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Figure 2. — Gfi1b overexpression represses Rag expression in primary cells. (A) Flow cytometric analysis of GFP levels in cultured bone marrow cells from RAG1-GFP heterozygous mice infected with a Thy1.1-marked retrovirus overexpressing either Gfi1b or an empty vector. Plots show populations gated according to B220, IgM, and Thy1.1 expression as indicated. Data are representative of five independent experiments. (B) Flow cytometric analysis of RAG1-GFP expression in IL-7–cultured bone marrow cells from RAG1-GFP heterozygous mice infected with a retroviral Gfi1 overexpression construct or an empty vector. This plot is representative of three different experiments. (C) Flow cytometric analysis of IgM expression in IL-7–cultured bone marrow cells from RAG1-GFP heterozygous mice infected with a retroviral Gfi1b overexpression construct or an empty vector. Data are representative of three independent experiments.