Figure 9.

The Gfi binding region within the RAG locus has a functional effect on RAG expression. (A) Reporter constructs used to assay RAG locus elements for effects on promoter activity. Erag, B cell–specific Rag locus enhancer; GBR, Gfi binding region; GFP, green fluorescent protein. (B) Flow cytometric analysis of GFP expression in AMuLV pro–B cells with stably integrated reporter constructs wherein GFP is driven by the indicated Rag locus DNA binding elements. Cells were analyzed in the absence or presence of a retroviral Gfi1b overexpression construct. Data are representative of two independent experiments.