FIG. 2.

Effect of inhibition of Prdx6 phospholipase A₂ (PLA2) activity by 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol (MJ33) on survival of WT and Prdx6 null PMVEC when subjected to peroxidative stress. (A) MJ33 inhibition of PLA₂ activity. WT PMVEC were treated with 0, 10, or 50 μM MJ33 for 30 min, and cells were harvested for PLA₂ activity assay. (B) MJ33 effect on survival of WT PMVEC. Cells were co-treated with 0, 10, 20, or 50 μM MJ33 and indicated concentrations of tBOOH for 24 h. (C) Comparison of MJ33 effect on WT and Prdx6 null PMVEC. Cells were co-treated with 0 or 50 μM MJ33 at the indicated concentrations of tBOOH for 24 h. The survival rates were measured by MTT assay. Data are expressed as mean±SE (n≥3). *p<0.05 compared with the corresponding control groups.