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. 2012 Mar 1;16(5):440–451. doi: 10.1089/ars.2011.3950

FIG. 3.

FIG. 3.

Efficiency of transfection of Prdx6 null PMVEC. (A) Transfection evaluated by green fluorescent protein (GFP) fluorescence. Prdx6 null PMVEC were transfected with pGFP-C1 vector or different pGFP-Prdx6 constructs by electroporation and incubated for 48 h. Upper panel shows phase images. Lower panel shows the green fluorescence from transfected cells. (B) Flow cytometry analysis of GFP fluorescence in transfected Prdx6 null PMVEC. Mock transfected cells (left panel) and WT GFP-Prdx6 transfected cells (right panel) were incubated for 72 h, and were then harvested for flow cytometry analysis. (C) Transfection evaluated by western blot. Prdx6 null cells were transfected with different pGFP-Prdx6 constructs and incubated for 72 h. The expression of GFP-Prdx6 proteins was analyzed by Western blot. Lane 1: Vector; Lane 2: WT Prdx6; Lane 3: S32A; Lane 4: H26A; Lane 5: D140A; Lane 6: C47S; Lane 7: S32A/C47S; Lane 8: D140A/C47S; Lane 9: D31A. All transfections were with a plasmid expressing a fusion protein with GFP. Results are representative of three experiments. (To see this illustration in color, the reader is referred to the Web version of this article at www.liebertonline.com/ars).