FIG. 7.

Rescue index for pGFP-Prdx6 constructs against 100 μM tBOOH induced peroxidative stress. To calculate rescue index, the survival rate for cells transfected with C1 vector was subtracted from survival rates for the cells transfected with Prdx6 constructs, then normalized to the transfection efficiencies indicated by secreted alkaline phosphatase activities. All transfections were performed with a plasmid expressing a fusion protein with GFP. Data are expressed as mean±SE (n≥3). (A) Rescue index measured by MTT assay (calculated from the experiments shown in Figs. 4 and 5). (B) Rescue index measured by the neutral red uptake assay. *p<0.05 compared with WT Prdx6. ^†p<0.05 compared with all single mutant constructs.