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Zyflamend modulates anti-apoptotic and pro-apoptotic protein expression. HCT-116 cells were treated with the indicated concentrations of Zyflamend for 24 h (A, C) or with 250 μg/mL Zyflamend for the indicated times (B, D). Whole-cell extracts were prepared and analyzed by Western blotting using the relevant antibodies. β-Actin was used as an internal control to verify equal protein loading.
