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. 2012 Mar 1;16(5):413–427. doi: 10.1089/ars.2011.3982

FIG. 3.

FIG. 3.

Zyflamend induces expression of death receptors. HCT-116 cells were treated with the indicated concentrations of Zyflamend for 24 h (A) or with 250 μg/mL Zyflamend for the indicated times (B). Whole-cell extracts were prepared and analyzed for DR5 and DR4 by Western blotting. β-Actin was used as an internal control to verify equal protein loading. (C) HCT-116 cells were treated with 100 μg/mL Zyflamend for 24 h and then harvested for analysis of cell surface expression of DR5 and DR4 by FACS. (D) Zyflamend up-regulated DR5 in various types of cancer cells. Cells were treated with the indicated concentrations of Zyflamend for 24 h, and whole-cell extracts were prepared and analyzed by Western blotting using antibodies against DR5 and DR4. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading. (Zy, Zyflamend) (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).