Table 2.
Correlation between Cytokines and CD68+ Macrophages or CD68+ CD163+ M2-type Macrophages
| Cytokines |
Test Applied | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IL-6 | IL-10 | bFGF | GM-CSF | IP-10 | MCP-1 | MIP-1α | RANTES | VEGF | MCP-3 | MIF | TRAIL | ICAM-1 | VCAM-1 | ||
| CD68+ macrophages with IHC | 0.33 | 0.73 | 0.60 | 0.22 | 0.32 | 0.23 | 0.41 | 0.06 | 0.25 | 0.03 | 0.54 | 0.11 | 0.41 | 0.61 | ANOVA |
| CD68+ macrophages with IF | 0.80 | 0.09 | 0.99 | 0.08 | 0.40 | 0.68 | 0.26 | 0.41 | 0.14 | 0.02 | 0.97 | 0.42 | 0.08 | 0.33 | Spearman's correlation |
| CD68+ CD163+ M2 type macrophages IF | 0.77 | 0.13 | 0.67 | 0.13 | 0.22 | 0.79 | 0.68 | 0.48 | 0.14 | 0.07 | 0.73 | 0.62 | 0.18 | 0.61 | Spearman's correlation |
Correlations between cytokines and CD68+ macrophages were determined using IHC in 37 cases and IF staining in 30 cases. Macrophages determined by IHC were categorized as low, medium, and high concentrations, whereas with IF, the macrophages were scored as the area (pixels) that stained positive. P-values shown in bold are significant.