Figure 5.

Src phosphorylation by IL-1β (0.01 ng/ml) in hippocampal neurons is dependent on AcPb. A, Dose–response treatment of IL-1β applied to cultured WT mouse hippocampal neurons for 20 min. Protein lysates were immunoblotted for p-Src and reprobed for total Src. Quantification of blots from three different experiments is shown below the blot. Densitometric values were expressed as p-Src/total Src, and then normalized to untreated cells (0 ng/ml). B, Time course of Src phosphorylation induced by IL-1β in mouse hippocampal neurons. At low concentration (0.01 ng/ml), Src is phosphorylated by IL-1β in WT neurons, but not in total AcP^−/− or AcPb^−/− neurons. Quantification of blots from three different experiments is shown below the blot. The significance was determined by one-way ANOVA with Tukey's post hoc analysis. Asterisks indicate values significantly different from control at *p < 0.05, ***p < 0.0001.