Table 1.
Echocardiographic and morphologic findings in mice from protocols I and II
| PROTOCOL I | PROTOCOL II | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT + Sham/VEH | WT MI + VEH | WT MI + ETN | WT + MI | TNF KO + Sham | TNF KO + MI | |||||||
| 24 h | 4 weeks | 24 h | 4 weeks | 24 h | 4 weeks | 24 h | 4 weeks | 24 h | 4 weeks | 24 h | 4 weeks | |
| n | 15 | 15 | 11 | 8 | 10 | 9 | 48 | 37 | 15 | 15 | 45 | 42 |
| B.Wt. | 23 ± 0.8 | 24.9 ± 1.2 | 22.8 ± 0.9 | 24.3 ± 1.7 | 23.2 ± 1.0 | 24.6 ± 1.3 | 22.9 ± 0.6 | 24.1 ± 1.2 | 21.7 ± 0.9 | 23.4 ± 1.2 | 22 ± 1.1 | 23.8 ± 0.8 |
| Echocardiographic parameters | ||||||||||||
| IVSS | 1.33 ± 0.03 | 1.41 ± 0.02 | 1.32 ± 0.01 | 1.43 ± 0.04* | 1.37 ± 0.06 | 1.35 ± 0.13 | 1.28 ± 0.08 | 1.39 ± 0.05* | 1.31 ± 0.08 | 1.33 ± 0.02 | 1.34 ± 0.08 | 1.36 ± 0.06 |
| IVSD | 0.84 ± 0.03 | 0.87 ± 0.05 | 0.81 ± 0.04 | 0.84 ± 0.04 | 0.82 ± 0.04 | 0.83 ± 0.06 | 0.79 ± 0.02 | 0.81 ± 0.07 | 0.76 ± 0.03 | 0.78 ± 0.02 | 0.79 ± 0.03 | 0.81 ± 0.04 |
| LVS | 2.62 ± 0.06 | 2.63 ± 0.08 | 2.85 ± 0.04 | 3.79 ± 0.12* | 2.87 ± 0.19 | 2.69 ± 0.12 | 2.87 ± 0.04 | 3.58 ± 0.13* | 2.51 ± 0.16 | 2.49 ± 0.11 | 2.62 ± 0.06 | 2.70 ± 0.11 |
| LVD | 4.17 ± 0.11 | 4.22 ± 0.15 | 4.11 ± 0.08 | 4.76 ± 0.12* | 4.17 ± 0.02 | 4.27 ± 0.07 | 4.17 ± 0.07 | 4.61 ± 0.08* | 3.98 ± 0.17 | 4.02 ± 0.16 | 4.16 ± 0.13 | 4.26 ± 0.15 |
| PWS | 1.23 ± 0.07 | 1.27 ± 0.06 | 1.34 ± 0.07 | 1.41 ± 0.04* | 1.24 ± 0.06 | 1.26 ± 0.10 | 1.19 ± 0.07 | 1.34 ± 0.08* | 1.25 ± 0.07 | 1.26 ± 0.07 | 1.28 ± 0.06 | 1.31 ± 0.06 |
| PWD | 0.75 ± 0.07 | 0.77 ± 0.04 | 0.82 ± 0.07 | 0.84 ± 0.05 | 0.78 ± 0.05 | 0.76 ± 0.09 | 0.78 ± 0.05 | 0.81 ± 0.08 | 0.79 ± 0.04 | 0.80 ± 0.06 | 0.76 ± 0.04 | 0.74 ± 0.04 |
| %FS | 37.3 ± 1.83 | 37.6 ± 0.24 | 30.6 ± 0.45 | 20.5 ± 0.70** | 30.9 ± 0.47 | 37.2 ± 1.10* | 31.1 ± 1.06 | 22.5 ± 0.79* | 37.1 ± 0.45 | 38.6 ± 0.32 | 36.9 ± 0.48 | 36.7 ± 1.01 |
| %IZ | N/A | N/A | 44.5 ± 2.3 | 47.6 ± 3.1 | 45.7 ± 3.2 | 48.6 ± 3.4 | 44.6 ± 2.9 | 47.2 ± 3.3 | N/A | N/A | 42.8 ± 2.8 | 45.6 ± 2.3 |
| Morphological parameters | ||||||||||||
| HW/BW | N/A | 4.33 ± 0.25 | N/A | 4.52 ± 0.05 | N/A | 4.29 ± 0.26 | N/A | 4.54 ± 0.18 | N/A | 4.21 ± 0.11 | N/A | 4.24 ± 0.13 |
| LW/BW | N/A | 4.61 ± 0.07 | N/A | 6.57 ± 0.36** | N/A | 4.49 ± 0.30 | N/A | 5.55 ± 0.34* | N/A | 4.31 ± 0.13 | N/A | 4.36 ± 0.16 |
Data presented are mean ± SEM
B.Wt (gm), body weight; IVD and IVS (mm), left ventricular internal diameter at end-systole and end-diastole, respectively; LVD and LVS (mm), left ventricular septal thickness at end-diastole and end-systole, respectively; PWD and PWS (mm), posterior wall thickness at end-diastole and end-systole, respectively; %FS, fractional shortening; %IZ, percent ischemic zone (* p < 0.05, ** p < 0.01 vs. 24 h MI); HW/BW, heart weight/body weight ratio; LW/BW, lung weight/body weight ratio.
*
p < 0.05,
**
p < 0.01 compared to respective sham group