Table 1.
The list of analyzed genes and selected PCR primers used in real-time PCR.
| Gene | Locus (AGI) | Forward primer (FP)Reverse primer (RP) | Tm FP or RP(°C) |
|---|---|---|---|
| THI1 | AT5G54770 | CTGCTGAGGATTTGATTGTG | 49,7 |
| CGATTGTGTGTGGTGGTT | 48,0 | ||
| THIC | AT2G29630 | TAGCTTACCACAAGGAGAA | 46,8 |
| CCAATGGAAAGAGCCACATCA | 52,4 | ||
| TH1 | AT1G22940 | GTGATCTTCCTGACTCAT | 45,8 |
| CCAAAGTGCAACCAGTCC | 50,3 | ||
| TPK | AT1G02880 | CACGATTCACTCCTCTTCTC | 51,8 |
| AATTCGTCGTAGATGCGATTAG | 52,0 | ||
| KGDH | AT3G55410 | TGCGTCAAACCGGGCTTGTT | 53,8 |
| AGTTGGCGGGAGTTGTGGCA | 55,9 | ||
| AT5G65750 | TGCTACCGTCGCTTTGGGCA | 55,9 | |
| TGAAGTGACGAGGGATGACTGCG | 58,8 | ||
| AT5G55070 | ACCGTTCTTGTCCAGGGTGTCCA | 58,8 | |
| AGGCGGCTAGGGTGCCATCA | 57,9 | ||
| PDH | AT5G50850 | TCGGTTTCGCCCTCAAGGCA | 55,9 |
| GTGGCTCGGTCAAGCGGACG | 60,0 | ||
| AT1G59900 | TGGTGTGAGGCAGGAACGGG | 57,9 | |
| AGAAGGCTCTGGCATTGGGCA | 56,3 | ||
| AT3G52200 | CGCCCGGACTTCCTCGCTTC | 60,0 | |
| GCACGCCAAATCGCAGAATGG | 56,3 | ||
| TK | AT3G60750 | GCCGTATGAGCCGTTCCAGGT | 58,3 |
| GAGCCTTCTCCCAGCCAGCA | 57,9 | ||
| AT2G45290 | ATTGGAGCCGTCACACGCCC | 58,3 | |
| TGGTGGCATCACCCGGAGAG | 57,9 | ||
| DXPS | AT4G15560 | TGCAAGCCATTGGACCGTGC | 55,9 |
| GTGCGGTTGCTGCGATGTGA | 55,9 | ||
| EF-1 | AT1G07920 | GAGCCCAAGTTTTTGAAGA | 46,8 |
| CTAACAGCGAAACGTCCCA | 51,1 | ||