Table 1.
Gene | Locus (AGI) | Forward primer (FP)Reverse primer (RP) | Tm FP or RP(°C) |
---|---|---|---|
THI1 | AT5G54770 | CTGCTGAGGATTTGATTGTG | 49,7 |
CGATTGTGTGTGGTGGTT | 48,0 | ||
THIC | AT2G29630 | TAGCTTACCACAAGGAGAA | 46,8 |
CCAATGGAAAGAGCCACATCA | 52,4 | ||
TH1 | AT1G22940 | GTGATCTTCCTGACTCAT | 45,8 |
CCAAAGTGCAACCAGTCC | 50,3 | ||
TPK | AT1G02880 | CACGATTCACTCCTCTTCTC | 51,8 |
AATTCGTCGTAGATGCGATTAG | 52,0 | ||
KGDH | AT3G55410 | TGCGTCAAACCGGGCTTGTT | 53,8 |
AGTTGGCGGGAGTTGTGGCA | 55,9 | ||
AT5G65750 | TGCTACCGTCGCTTTGGGCA | 55,9 | |
TGAAGTGACGAGGGATGACTGCG | 58,8 | ||
AT5G55070 | ACCGTTCTTGTCCAGGGTGTCCA | 58,8 | |
AGGCGGCTAGGGTGCCATCA | 57,9 | ||
PDH | AT5G50850 | TCGGTTTCGCCCTCAAGGCA | 55,9 |
GTGGCTCGGTCAAGCGGACG | 60,0 | ||
AT1G59900 | TGGTGTGAGGCAGGAACGGG | 57,9 | |
AGAAGGCTCTGGCATTGGGCA | 56,3 | ||
AT3G52200 | CGCCCGGACTTCCTCGCTTC | 60,0 | |
GCACGCCAAATCGCAGAATGG | 56,3 | ||
TK | AT3G60750 | GCCGTATGAGCCGTTCCAGGT | 58,3 |
GAGCCTTCTCCCAGCCAGCA | 57,9 | ||
AT2G45290 | ATTGGAGCCGTCACACGCCC | 58,3 | |
TGGTGGCATCACCCGGAGAG | 57,9 | ||
DXPS | AT4G15560 | TGCAAGCCATTGGACCGTGC | 55,9 |
GTGCGGTTGCTGCGATGTGA | 55,9 | ||
EF-1 | AT1G07920 | GAGCCCAAGTTTTTGAAGA | 46,8 |
CTAACAGCGAAACGTCCCA | 51,1 |