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The NAg domain of murine GMCSF-NAg TTV did not interfere with cytokine bioactivity. Designated concentrations (x-axis) of murine GM-CSF, rat GM-CSF, murine GMCSF-MOG, or murine GMCSF-PLP were cultured with C57BL/6 bone marrow cells (100,000 cells per well) (A) or FDC-P1 cells (10,000 cells per well) (B). Cultures were pulsed with 1 uCi of [3H]thymidine (A) or MTS/PMS (B) during the last 24 hours of a 3-day culture. These data are representative of four experiments.
