Figure 2. H2R is necessary for IL-4 induced eosinophil recruitment and CCL24 production.
5 μg of IL-4 or BSA was administered intratracheally for 3 consecutive days to WT or H2R KO mice. On day 4, total cells (A), eosinophils (B) and CCL24 levels (C) in BAL fluid were determined. Data represent mean ± SEM from 3 independent experiments (n = 7-9). 5 μg of IL-4 or BSA was administered intratracheally for 3 consecutive days to HDC KO mice with or without histamine (10μM) or dimaprit and total cells (D), eosinophils (E) and CCL24 levels (F) in BAL fluid were measured on day 4. Data represent mean ± SEM from 3 independent experiments (n = 4-6). *p < 0.05; **p < 0.05; *** p < 0.005 by Students t-test.