Figure 3. Bone marrow derived CX₃CR1+ cells are drivers of tumor angiogenesis.

(A) FACS analysis of cx₃cr1 ^gfp cells purified by FACSAria Cell-Sorting System from BM of CCR2+ CD45.1 donor mice before their transfer to CCR2−/− mice (B) Imaging (IVIS) of the primary tumor on day 60, as recorded by the IVIS camera using – luciferin filter (recording luciferase activity of the cancer cells) as follows: CCR2+/+ C57BL/6 mice (WT) (a), CCR2−/− mice (b), CCR2−/− transplanted with GFP+ cells from BM of CCR2+ donor mice (c). All photos show a representative mouse per group (1 of 6 mice). (C) Computerized CCCD analysis of six mice per group. Results of six mice per group are shown as total flux (p/s ×10⁴) ±SE. * Indicates p<0.001. (D) Representative primary tumor sections were then analyzed by to immunostaining using different colors for CD45.1 (red color, only transferred cx₃cr1 ^gfp cells) and CD11b+ (green). (E) Analysis of 60 sections from six mice per group for the relative number of CD11b+ cells at tumor sections from each group, and of CD45.1 cells following cell transfer * Indicates p<0.001.