Figure 4. RKIP silencing induces nuclear translocation of NRF2.

A, Shows western blotting and densitometric measurements (upper panel) for NRF2, RKIP and β-actin loading control from total protein extracts of depicted cells. B, Shows western blots on nuclear fractions of HEK-control and HEK-499 cells in duplicates with Aurora B as loading control. C, Immunofluorescent confocal microscopy for NRF2 in the depicted cells showing increased nuclear localization of NRF2 in RKIP depleted HEK-499 cells. Western experiments were performed in duplicates and repeated twice.