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. 2012 Jan 18;7(1):e29654. doi: 10.1371/journal.pone.0029654

Figure 2. OTUB1 and DCN mRNA were upregulated in MC stimulated with IL-1βand ATS.

Figure 2

(a) MC were treated with IL-1β10 ng/mL for 3 h, 6 h, 12 h and 24 h. (b) MC were treated with ATS 30 µL/mL for 3 h, 6 h, 12 h and 24 h respectively. mRNA levels of OTUB1 and DCN in MC were analyzed by quantitative real-time PCR. Besides the group of OTUB1 in ATS treating for 3 h, both OTUB1 mRNA and DCN mRNA significantly increased in all groups treated with IL-1β or ATS compared with normal group (*P<0.05, **P<0.01). Data are presented as mean±standard error (SE) from at least three individual experiments carried out in duplicate.