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. 2012 Jan 18;7(1):e29654. doi: 10.1371/journal.pone.0029654

Figure 5. Overexpression of OTUB1 downregulated the expression of DCN in MC.

Figure 5

(a) mRNA expression of OTUB1 in MC after OTUB1 gene transfection was assessed by RT-PCR. Lane 2, 5, 8 are normal mesangial cells (MC) as control, Lane 3, 6, 9 are pIRES2-EGFP-OTUB1-myc stable transfected MC (MC/OTUB1), Lane 4, 7, 10 are vacant vector pIRES2-EGFP transfected MC (MC/pIRES2). The mRNA expression of OTUB1 increased in lane 9. (b) Protein expression of OTUB1 and DCN were assessed by Western blot. Lane 1 is normal mesangial cells (MC) as negative control, Lane 2 is pIRES2-EGFP-OTUB1-myc stable transfected MC (MC/OTUB1), Lane 3 is vacant vector pIRES2-EGFP transfected MC (MC/pIRES2). Overexpression of OTUB1 was seen in lane 2 with myc labeling positive. Meanwhile, the DCN expression significantly decreased after OTUB1 gene transfection in lane 2. (□) OTUB1/β-actin; (▪ )DCN/β-actin. (*P<0.05; **P<0.01). Data are presented from at least three individual experiments carried out in duplicate.