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. 2012 Jan 18;7(1):e29812. doi: 10.1371/journal.pone.0029812

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Della Santina et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: HCN channel 1–2 mRNA expression in murine retina. The amount of HCN amplicons is compared to cyclophilin expression. B: Confocal images of retinal sections immunolabeled with rabbit polyclonal antibodies (green fluorescence) specific for HCN1 (upper panel) and HCN2 (bottom panel) in HCN^+/+, HCN1^−/− and HCN2^−/− mice. In addition to immunolabeling with the antibody for HCN2 (bottom panel), the retinas were also stained with an antibody against PKC, a specific marker for rod bipolar cells (red fluorescence). Scale bars, 10 µm.