Figure 5. WASp is not required for the recruitment of PI 3-kinase, Rac1, WAVE2 and actin in CSF-1-elicited protrusions from primary BMMs.

Chemotactic protrusions were isolated from wild-type and WASp^−/− BMMs in response to CSF-1 as described in Materials and Methods. (A) Representative western blots of indicated proteins from pseudopod lysates and total cell lysates are shown. (B) Protein amounts from pseudopod lysates were quantified by densitometry and normalized to the corresponding level of a non-specific membrane marker (CD14). Data is shown as relative ratio of each proteins compared to the WT BMM. n = 3 experiments. Error bars represent SEM.