Table 1. Summary of micropipette assay chemotactic responses.

Macrophages	Directional protrusion (%)	Retraction (%)	n (experiments; cells)
shControl	94.8±3.4	43.2±13.1	7; 49
shWASp	91.1±3.3	78.7±7.1*	7; 49
Y291F	93.8±3.2	23.3±10	10; 36
WT BMM	91.0±3.9	4.5±3.1	13; 32
WASp−/− BMM	89.7±4.3	81.7±6.67***	10; 33

Percentages of cells that exhibited directional protrusions towards the micropipette and retracted within five minutes of CSF-1 stimulation are shown. Percentages were calculated by dividing the number of positive responses over the total number of cell responses recorded from each of the analyzed time-lapse images and averaging them. Indicates SEM. n indicates the total number of experiments and cells analyzed for each macrophage type.

*indicates p<0.05 compared to shControl and Y291F cells.

***indicates p<0.001 compared to wild type (WT) BMM.