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. 2012 Jan 18;7(1):e30571. doi: 10.1371/journal.pone.0030571

Figure 4. Signal transduction in the hypothalamus.

Figure 4

Hypothalamic total protein extracts obtained from Swiss mice fed on regular chow (CT), high-fat diet (HF), flax seed- (FS) or olive oil- (OL) substituted (10, 20 or 30%) diets for eight weeks were used in immunoblotting (IB) experiments to evaluate protein expression and/or activity. Specific antibodies against phospho-IκB-α (P-IκBα) (A), phospho-JNK (P-JNK) (B), TNF-α (C), SOCS-3 (D), iNOS (E), IL-10 (F), Caspase-3 (CASP-3) (G), BAX (H), Bcl-2 (I), phospho-ACC (P-ACC) (J), FAS (K) and CPT-1 (L) were used to identify respective protein targets. Loading was evaluated by re-probing the membranes with anti-β-actin (A, C-I, K and L), anti-JNK (B) or anti-ACC (J) antibodies. In all experiments, n = 5; #p<0.05 vs. CT and *p<0.05 vs. HF.