Table 2.

In vivo kinetics of killing of C.elegans infected by E. coli grown in urine samples after consumption of the different proanthocyanidins plus propolis regimens.

Strains	PACs mg	Propolis mg	Collection time	LT50	LT100	p
						T0 vs. others	test 1 vs. test 2	test 1 vs. test 4
NECS978323	-	-	0	4.2 ± 0.2	7.4 ± 0.6	NA
test 1	60	-	4-6 h	6.5 ± 0.1	11.5 ± 0.5	< 0.001	NS
	60	-	12 h	5.6 ± 0.2	9.5 ± 0.5	< 0.001	NS
	60	-	24 h	4.2 ± 0.2	7.3 ± 0.7	NS	NS
test 2	60	170	4-6 h	6.0 ± 0.1	11.3 ± 0.7	< 0.001	NS
	60	170	12 h	5.5 ± 0.2	9.3 ± 0.7	< 0.001	NS
	60	170	24 h	5.0 ± 0.2	7.8 ± 0.8	NS	NS
test 3	60	192	4-6 h	6.1 ± 0.1	11.2 ± 0.8	< 0.001
	60	192	12 h	5.5 ± 0.2	9.5 ± 0.5	< 0.001
	60	192	24 h	5.3 ± 0.2	8.5 ± 0.5	0.02
test 4	90	255	4-6 h	6.3 ± 0.3	11.3 ± 0.7	< 0.001		NS
	90	255	12 h	5.3 ± 0.3	9.5 ± 0.5	< 0.001		NS
	90	255	24 h	5.8 ± 0.2	9.0 ± 1.0	< 0.001		< 0.001
test 5	120	340	4-6 h	6.0 ± 0.1	11.8 ± 0.8	< 0.001
	120	340	12 h	6.2 ± 0.2	11.8 ± 0.8	< 0.001
	120	340	24 h	6.3 ± 0.3	11.5 ± 0.5	< 0.001
test 6	-	-	4-6 h	4.1 ± 0.2	7.2 ± 0.8	NS
	-	-	12 h	3.9 ± 0.1	7.0 ± 0.5	NS
	-	-	24 h	4.2 ± 0.3	7.3 ± 0.7	NS
OP50	-	-	-	7.5 ± 0.5	13.3 ± 0.7	< 0.001

The results are representative of at least three independent assays. OP50 is an avirulent E. coli strain used as control of the vitality of worms. E. coli NECS978323 was the strain used in the study. It was used as control (without treatment)

NS not significant