Table 3.

Evaluation of the number of bacteria within the C.elegans digestive tract.

Strains	PACs mg	Propolis mg	Collection time	Median CFU [range]/nematodes after 72 h
NECS978323	-	-	0	8.1 × 105 [7.0-9.3 106]
test 1	60	-	4-6 h	3.3 × 105 [2.5-4.0 106]
	60	-	12 h	3.5 × 105 [2.1-5.0 106]
	60	-	24 h	5.2 × 105 [4.4-6.1 106]
test 2	60	170	4-6 h	4.4 × 105 [4.0-4.8 106]
	60	170	12 h	5.1 × 105 [4.8-5.4 106]
	60	170	24 h	6.0 × 105 [4.9-7.2 106]
test 3	60	192	4-6 h	2.3 × 105 [2.0-3.8 106]
	60	192	12 h	4.8 × 105 [4.2-5.4 106]
	60	192	24 h	3.0 × 105 [2.5-3.5 106]
test 4	90	255	4-6 h	2.7 × 105 [1.0-4.2 106]
	90	255	12 h	4.3 × 105 [3.5-5.1 106]
	90	255	24 h	3.7 × 105 [3.2-4.0 106]
test 5	120	340	4-6 h	2.5 × 105 [1.9-3.0 106]
	120	340	12 h	3.8 × 105 [2.2-5.4 106]
	120	340	24 h	1.8 × 105 [1.0-3.1 106]
test 6	-	-	4-6 h	6.0 × 105 [4.9-7.2 106]
	-	-	12 h	4.5 × 105 [3.9-5.1 106]
	-	-	24 h	7.1 × 105 [6.8-7.5 106]
OP50	-	-	-	6.8 × 105 [6.0-7.5 106]

After 72 h of infection, the C. elegans were washed and ground, and dilutions of the resulting suspension were plated on selective media. The number of CFU per worm of the different strains alone or in combination was calculated. Three replicates were performed for each bacterial combination. E. coli NECS978323 was the strain used in the study. It was used as control (without treatment). No statistical difference could be demonstrated

Range of values are indicated into [square brackets]