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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Oral Oncol. 2011 Oct 19;48(1):26–32. doi: 10.1016/j.oraloncology.2011.09.010

Figure 1.

Figure 1

ALDH and CD44 identify cells with stem-like phenotype in vitro. (A) Representative phase contrast images of orospheres developed from ALDH+CD44+ (cancer stem-like cells) or ALDH−CD44− (control cells) sorted from the primary tumor-derived (UM-SCC-22A) or from the metastatic lymph node-derived (UM-SCC-22B) cell lines. Cells were cultured in ultra-low attachment plates for 7 days. (B) Graph depicting the quantification of the number of orospheres. Statistical significance was determined at P<0.05 and are indicated by different low case letters. (C) Graph depicting the proliferation of ALDH+CD44+ or ALDH−CD44− cells, as determined by the WST-1 assay.