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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Oral Oncol. 2011 Oct 19;48(1):26–32. doi: 10.1016/j.oraloncology.2011.09.010

Figure 3.

Figure 3

VEGF secreted by endothelial cells enhances the proliferation of primary, not metastatic, HNCSC. (A–D) Graph depicting the proliferation of ALDH+CD44+ or ALDH−CD44− cells, as determined by the WST-1 assay. Cells were cultured in serum-free conditioned medium collected from primary endothelial cells (EC CM) supplemented with 1 μg/ml anti-VEGF antibody or non-specific isotype-matched IgG (control) in ultra-low attachment plates for 48 hours. As additional controls, we evaluated cells cultured in serum-free endothelial basal medium (EBM) supplemented (or not) with 50 ng/ml rhVEGF165.