Expression of ASP and ROPN1 from homozygous wild type (WT, lane 1), homozygous Asp gene trap (AGT, lane 2), or homozygous Ropn1 gene trap (RGT, lane 3) mice. Western blots using rabbit polyclonal antibodies against ASP (top panel), ROPN1 (middle panel) and AKAP3 (lower panel) were performed. The western blots pictured here are representative of at least three independent experiments, on at least three unique sets of mice.