Figure 6. Ciliary alignment and axonemal ultrastructure are intact in mice that lack ASP and ROPN1.

Trachea from wild type (WT), Asp^GT (A^GT), Ropn1^GT (R^GT), and Asp^GT/Ropn1^GT (A^GT/R^GT) mice were harvested and sectioned into rings prior to fixation and preparation for transmission electron microscopy (see Materials and Methods for details). In A and B, to assess whether cilia lacking ASP and/or ROPN1 were disoriented, a layer was created over the EM image (in Photoshop), and lines were drawn through the central pair of each axoneme. The layer was then offset so that central pairs would be visible in the presented images. Asterisks in corresponding upper and lower panels provide orientation. In C, a close up of an Asp^GT/Ropn1^GT (A^GT/R^GT) cilium showing that major ciliary structural components (as labeled in the figure) are intact in the absence of ASP and ROPN1.

In D, a diagram and an image of a wild type central pair apparatus (upper panel) and images of central pair apparatuses from flagellar axonemes that lack ASP [A^GT (lower panels), shows two raw axonemal images (left), and a merged image of the two axonemes with central pair projections labeled (right, merge performed in Photoshop)]. Scale bars are 250 nm.