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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Methods. 2011 Sep 16;56(2):154–160. doi: 10.1016/j.ymeth.2011.09.005

Figure 5.

Figure 5

Inter-bead independence is evident by comparing single- to multiplex IP-FCM. IP beads specific for the primary analyte indicated on the x-axis were incubated with TOT-1 cell lysate. For this experiment, 2 × 103 beads were incubated with cell lysate consisting of 1.75 × 106 cells/0.02 mL lysis volume (2.3 × 10−5 beads per cells/mL). Captured TCR complexes were stained for co-associated TCRβ, with resulting fluorescence depicted on the y-axis. Black bars, IP-FCM performed in singleplex using the single IP bead indicated. White bars, IP-FCM performed in multiplex using the combination of the five IP beads listed.