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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Methods. 2011 Sep 16;56(2):154–160. doi: 10.1016/j.ymeth.2011.09.005

Figure 6.

Figure 6

Confirmation of AAC via analyte non-depletion using sequential multiplex IP-FCM. A multiplex IP bead set specific for the primary analytes indicated on the x-axis were incubated with TOT-1 cell lysate. For this experiment, 2 × 103 beads were incubated with cell lysate consisting of 1.75 × 106 cells/0.02 mL lysis volume (2.3 × 10−5 beads per cells/mL). After this first IP (black bars), the post-IP lysate was then subjected to a second round of IP with fresh multiplex IP-FCM beads (white bars). Captured TCR complexes were stained for co-associated TCRβ, with resulting fluorescence depicted on the y-axis.