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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Traffic. 2011 Dec 4;13(2):305–316. doi: 10.1111/j.1600-0854.2011.01310.x

Figure 6. Loss of APβ1/2 leads to disruption of the contractile vacuole network.

Figure 6

Cells transformed with the contractile vacuole marker Dajumin-GFP were incubated in water and examined by fluorescence microscopy. Dajumin-labeled contractile vacuoles are seen in wild-type cells and cells deficient in the alpha (α) and medium (mu2) subunits of the AP2 complex. No contractile vacuoles were detected in 100% (n=110, 3 independent experiments) of cells deficient in APβ1/2 (β-), similar to μ1 or clathrin heavy chain mutants. Scale bar, 10μm.