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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Cancer Res. 2011 Nov 29;72(2):472–481. doi: 10.1158/0008-5472.CAN-11-1628

Figure 5. Effect of JNK-deficiency on mammary gland development.

Figure 5

Transplantation assays were performed using mammary tissue from tamoxifen-treated female donor mice and female recipient nude mice. Control (CreERT) tissue was transplanted in one cleared mammary gland and Jnk1Δ/Δ Jnk2−/− CreERT tissue was transplanted in the contralateral gland of the same recipient mouse.

A–C) Whole mount mammary glands stained with carmine alum at 8 weeks following transplantation are shown (A,C). The number of branches per unit length (mm) of duct is presented as the mean ± SD; n = 3 (B). Statistically significant differences are indicated with an asterisk (*, p < 0.05). Scale bars: 5 mm (A); 200 µm (C).

D–F) Terminal end buds in whole mount mammary glands stained with carmine alum at 2 weeks following transplantation are shown (D). Stained sections of the end buds in whole mount mammary glands are presented (E). The number of branches per unit length (mm) of duct is presented as the mean ± SD; n = 3 (F). Statistically significant differences are indicated with an asterisk (*, p < 0.05). Scale bars: 200 µm (D); 100 µm (E).