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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Bone. 2011 Aug 18;50(2):477–489. doi: 10.1016/j.bone.2011.08.010

Figure 1. Wnt6, Wnt10a and Wnt10b mRNA expression decreases during adipogenesis in vivo and in vitro.

Figure 1

(A) Epididymal WAT from C57BL/6J mice was separated into stromovascular (SVF) and Adipocyte fractions. Total RNA was isolated from each fraction, and the expression of FABP4, PPARγ, Wnt10b, Wnt10a and Wnt6 was analyzed by qPCR and normalized to 18S rRNA. Data are presented relative to the maximum expression for each transcript as mean ± SD (n = 5 or 6). (B-E) ST2 bipotential cells (B, D) or 3T3-L1 preadipocytes (C, E) were induced to differentiate into adipocytes. (B, C) Adipogenesis was assessed by staining with Oil Red-O at day 0 or days 6-8 post-induction. Micrographs of stained cells, representative of at least three independent experiments, are shown. (D, E) Total RNA was isolated at the indicated time points and the transcript expression of Wnt6, Wnt10a and Wnt10b was analyzed by qPCR and normalized to 18S rRNA. Data are presented relative to expression at day 0 as mean ± SD of three independent experiments. Statistically significant differences between SVF and Adipocytes (A) or compared to transcript levels at day 0 (D, E) are indicated as follows: * = P < 0.05; ** = P < 0.01; *** = P < 0.001.