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Enzyme activity of APN present on HT-1080 cells in the presence of increasing concentrations of bestatin or ReO-N3S-Probestin conjugates. The enzyme activity was estimated by measuring the absorbance at 405 nm of p-nitroaniline formed by the hydrolysis of L-alanine-p-nitroanilide (substrate) at room temperature in 30 min. EC50 values were calculated by non-linear regression (dose-response, variable slope) analysis using GraphPad Prism.
