Fig. 2.

E-4031 depolarizes the resting potential. a Recording of resting potential in a SW2 cell. Application of 3 μM E-4031 induced a depolarization which was not reversible within the recording time. b Superimposed tail current recordings elicited with a pulse to −100 mV, preceded by 4-s test pulses to potentials between 20 and −80 mV from a holding potential of −80 mV. Extracellular solution: Ringer’s with 5 mM K⁺. c Activation curve. Tail current amplitudes as shown in b were normalized (n = 6), averaged, plotted versus the preceding test pulse potential and fitted with a Boltzmann function, yielding V _0.5 = −18.5 mV and k = 11.9 mV