Table 1.
E-4031 does not affect cell proliferation
| Cell line | IHERG (pA/pF), n/n+ | Cell proliferation | |||
|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | |||
| SW2 | 31.7 ± 2.5, 22/25 | Control, 1 μM E-4031; 5 μM E-4031 | 1.00 ± 0.05; 0.92 ± 0.03; 0.99 ± 0.02 | 1.00 ± 0.05; 0.86 ± 0.04; 0.90 ± 0.06 | 1.00 ± 0.03; 0.89 ± 0.06; 0.84 ± 0.04 |
| OH1 | 13.2 ± 2.8, 5/7 | Control, 1 μM E-4031; 5 μM E-4031 | 1.00 ± 0.01; 0.98 ± 0.02; 1.00 ± 0.05 | 1.00 ± 0.06; 0.96 ± 0.06; 0.84 ± 0.05 | 1.00 ± 0.02; 0.96 ± 0.02; 0.92 ± 0.05 |
| OH3 | 34.5 ± 2.7, 4/6 | Control, 1 μM E-4031; 5 μM E-4031 | 1.00 ± 0.03; 0.97 ± 0.04; 0.92 ± 0.01 | 1.00 ± 0.08; 0.93 ± 0.07; 0.92 ± 0.09 | 1.00 ± 0.05; 0.85 ± 0.03; 0.91 ± 0.05 |
| H82 | 12.7 ± 0.8, 3/5 | Control, 1 μM E-4031; 5 μM E-4031 | 1.00 ± 0.02; 1.04 ± 0.14; 0.97 ± 0.09 | 1.00 ± 0.10; 1.03 ± 0.18; 1.37 ± 0.10 | 1.00 ± 0.05; 1.11 ± 0.20; 1.09 ± 0.13 |
Cell proliferation of four SCLC cell lines (SW2, OH1, OH3, H82) measured after 24, 48, and 72 h in the presence of 1 or 5 μM E-4031 normalized to the corresponding control cells in the absence of E-4031 (mean ± SEM). Each mean value was calculated from six to nine measurements. There was no statistically significant change in cell proliferation as compared with the control cells by using the t-test and the Bonferroni correction
n number of cells containing HERG current, n+ total number of cells investigated