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. 2011 Sep 10;101(2):347–357. doi: 10.1007/s10482-011-9640-8

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© The Author(s) 2011

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Fig. 3 — Fluorescence of Beggiatoa strain 35Flor filaments obtained by dual staining with DAPI for polyphosphate and the pH-sensitive dyes LysoSensor Green DND-189 or Acridine Orange. a LysoSensor Green DND-189 shows fluorescence mostly in the cell periphery, indicating a low pH. b DAPI reveals polyphosphate inclusions of different sizes in the same filament. c The overlay of (a) and (b) shows no fluorescence for LysoSensor Green DND-189 within polyphosphate inclusions, indicating that the pH is not notably acidic. d Acridine Orange reveals several spheres of acidic pH in the cell periphery. e DAPI reveals polyphosphate inclusions of different sizes in the same filament. f The overlay of (d) and (e) reveals no fluorescence for Acridine Orange in polyphosphate inclusions, indicating that the pH in the polyphosphate inclusions is not notably acidic. Scale bars represent 5 μm