Table 7.
The frequency of MN in human lymphocytes treated with Sp alone or with Sp+Cyp in the presence of S9mix
| Test substance | Concentration (μL/mL) | Micronucleated binuclear cell (%) ± SE |
|---|---|---|
| Control | – | 0.250 ± 0.050 |
| Methanol | 6.0 | 0.500 ± 0.100 |
| Cyp | 28 μg/mL | 1.280 ± 0.120d |
| Sp | 1.5 | 0.525 ± 0.075 |
| 3.0 | 1.125 ± 0.075 a1 | |
| 6.0 | 0.800 ± 0.050 a1 | |
| Sp+Cyp | 1.5 Sp+Cyp* | 1.000 ± 0.090 a1 |
| 3.0 Sp+Cyp* | 1.080 ± 0.080 a1e | |
| 6.0 Sp+Cyp* | 0.775 ± 0.045 c1 |
a: significant from control; b: significant from methanol control; c: significant from positive control, Cyp; a1b1: P < 0.05; a2b2: P < 0.01
* Cyp (Cyclophosphamide): 28 μg/mL
d: 3,713 and e: 3,183 cells were scored for excessive toxicity