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. 2012 Jan 5;2012:683065. doi: 10.1155/2012/683065

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Copyright © 2012 A. Dutour et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cytotoxicity of anti-CD33.CAR-transduced EBV-CTLs against normal myeloid progenitors. (a) Unmanipulated or anti-CD33.CAR-transduced EBV-CTLs were incubated with bone-marrow-derived CD34⁺ progenitors at E : T ratio of 1 : 1 for 4 hours. Cells were then seeded in methylcellulose-based medium and after 10 days CFU-GM, CFU-GEMM, and BFU-E were counted. Data shown are mean ± SD of 6 independent experiments. (b) Colonies were collected and characterized by flow cytometry to assess the expression of surface markers specific for each colony type. Data shown are mean ± SD of 6 independent experiments.