Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| S. aureus SH1000 | S. aureus strain 8325-4 with functional RsbU | [41] |
| S. aureus RN4220 | A restriction minus derivative of S. aureus 8325-4 | [42] |
| VKS1009 | SH1000 with msrA1/B-lacZ integration (Ermr) | This study |
| VKS1010 | SH1000 with msrA2-lacZ integration (Ermr) | This study |
| VKS1011 | SH1000 with msrA3-lacZ integration (Ermr) | This study |
| E. coli JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1thi Δ(lac-proAB) F′(traD36proAB+laclqΔM15) | [43] |
|
| ||
| Plasmids | ||
| pGEMT | Cloning vector for E. coli (Ampr) | Promega |
| pAZ106 | lacZ fusion vector (Ampr, E. coli; Ermr, S. aureus) | [27] |
| pGEMT-msrA2P | pGEMT containing the msrA2 promoter fragment | This study |
| pGEMT-msrA3P | pGEMT containing the msrA3 promoter fragment | This study |
| pAZ-msrA2P | pAZ106 containing msrA2 promoter-lacZ fusion | This study |
| pAZ-msrA3P | pAZ106 containing msrA3 promoter-lacZ fusion | This study |
Ermr: erythromycin resistant, Ampr: ampicillin resistant.