Figure 4.
SEM and elemental mapping of PILP-remineralized manatee bone treated with 100 μg/ml of 10-KDa poly-L-aspartic acid for 7 days. (Top row) Cross-section of PILP-remineralized bone sample. (Bottom row) A closer look at an osteon found at the cross-section of the PILP-remineralized bone sample. (A, E) SEM showing the cross-sectional morphology of the sample. (B, F) Backscattered electron mode images of the same region. These images display more electron-dense areas at the edges and surrounding the osteonal structures of the specimen. Elemental mapping showing calcium (red) and phosphorus (green) distribution across the sample (C, G) and (D, H) respectively.