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. 2011 Nov 1;31(2):267–278. doi: 10.1038/emboj.2011.395

Figure 2.

Figure 2

Association of TPR proteins and HSP90 with AGO1. FLAG–AGO1 and N-terminally myc-tagged TPR proteins were synthesized in BYL, mixed, and incubated at 25°C for 30 min in the presence of additional 1 mM MgCl2 and 2% DMSO (−ATPγS −GA); 0.75 mM ATPγS, 1 mM MgCl2 and 2% DMSO (+ATPγS −GA); or 0.75 mM ATPγS, 1 mM MgCl2, 2% DMSO, and 20 μM GA (+ATPγS +GA). The myc–TPR proteins synthesized in BYL were also mixed with mock-translated BYL and incubated in the [+ATPγS −GA] condition. The samples before (input) and after (FLAG-IP) immunopurification with anti-FLAG antibodies were analysed by immunoblotting using anti-myc, anti-HSP90, and anti-AGO1 antibodies.